Fig. 2

MST1 Overexpression aggravates cognitive impairment in 5-month 5xFAD mice. A Schematic diagram of experimental arrangement. AAV-GFP-vehicle and AAV-GFP-MST1 was injected into the hippocampus of 4-month C57 mice and 5xFAD mice, and then the mice were divided into four groups: C57 + AAV vehicle, C57 + AAV MST1, 5xFAD + AAV vehicle, and 5xFAD + AAV MST1 for experiments. One month later, MWM test was performed, and finally all mice were euthanized for other experiments. B Representative spontaneous fluorescence images of the AAVs-infected slices (n = 4, Scale bar is 2.5 µm). C Representative immunoblotting bands of hippocampus MST1 and p-MST1 after AAVs injection. D Quantitative analysis of MST1 and p-MST1 in hippocampus (n = 4). E MST1 mRNA levels in the hippocampus after AAVs injection were detected using RT-qPCR (n = 3). F-J MWM test results of four groups of mice, including swimming speed (F), escape latency (G), total time spent in the target quadrant (H), number of platform crossings (I), representative traces on fifth day the MWM training period (J). (n = 12). All data represent means ± SEMs. *, Comparison between C57 + AAV-vehicle and 5xFAD + AAV-vehicle group, *p < 0.05; #, Comparison between 5xFAD + AAV-vehicle and 5xFAD + AAV-MST1 group, #p < 0.05; ##p < 0.01; ###p < 0.001