Fig. 4
From: Deletion of MAPL ameliorates septic cardiomyopathy by mitigating mitochondrial dysfunction
MAPL deficiency prevented CLP-induced mitochondrial fission. A. Transmission electron microscopy images of H9C2 cardiomyocytes after MAPL knockdown. Yellow arrows indicate abnormal mitochondria in the cardiomyocytes. B-D. Quantification of mitochondria-related parameters from A. B. Mitochondrial aspect ratio refers to the ratio of mitochondrial maximum diameter to minimum diameter (**P < 0.001, ****P < 0.0001, n = 6 repeated experiments). C. Mitochondrial number/µm2 refers to the ratio of mitochondrial number to image area (***P < 0.001, n = 6 repeated experiments). D. Mitochondrial cristae numbers/µm2 refers to the ratio of intact mitochondrial cristae to image area (****P < 0.0001, n = 6 repeated experiments). E. Transmission electron microscopy images of WT and MAPL CKO mice heart tissues after sham or CLP procedures. F, G and H. Quantification of mitochondria-related parameters from E. F. Mitochondrial aspect ratio refers to the ratio of mitochondrial maximum diameter to minimum diameter (****P < 0.0001, n = 6 for each group). G. Mitochondrial num/µm2 refers to the mitochondrial number to the image area (****P < 0.0001, n = 6 for each group). H. Abnormal mitochondrial number refers to abnormal mitochondria to image area (****P < 0.0001, n = 6 for each group). I. ATP concentration in heart tissue of mice was determined after CLP or sham surgery (*P < 0.05, **P < 0.001, n = 5 for each group). J. ATP concentration in H9C2 cardiomyocytes was determined after MAPL knockdown (**P < 0.01, n = 5 repeated experiments). K. MMP of shNC-and shMAPL-cardiomyocytes treated with LPS (20 µg/ml) was detected by JC-1 kit (green: monomer, red: aggregates, CCCP was used as a positive control, scale bar = 10 μm, n = 5 repeated experiments). L. The ROS level in shNC- and shMAPL-cardiomyocytes treated with LPS (20 µg/ml) was detected by DCFH-DA staining. Scale bar = 10 μm (****P < 0.0001, n = 5 repeated experiments)