Fig. 3
From: Deletion of MAPL ameliorates septic cardiomyopathy by mitigating mitochondrial dysfunction
MAPL deficiency alleviates apoptosis of cardiomyocytes during SCM. A. The protein levels of Bcl-2, Bax, and cleaved-caspase 3 in shNC and shMAPL cardiomyocytes after LPS (20 µg/ml) treatment were detected by western blot (**P < 0.01, ***P < 0.001, ****P < 0.0001, n = 5 repeated experiments). B. The protein levels of Bcl-2, Bax, and cleaved-caspase 3 of heart tissues from WT and CKO mice after sham surgery or CLP were detected by western blot (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n = 5 for each group). C. Immunofluorescence staining was performed to detect the expression of Bax in shNC and shMAPL cardiomyocytes after LPS (20 µg/ml) treatment (scale bar = 10 μm. n = 5 repeated experiments). D. TUNEL staining of shNC and shMAPL cardiomyocytes after LPS (20 µg/ml) treatment was detected by TUNEL BrightGreen Apoptosis Detection Kit (scale bar = 10 μm. n = 5 repeated experiments)