Fig. 5

Exogenous mitochondria promoted MG/MΦ polarization to M2-type. (A)-(D) Immunoblotting analysis of hippocampal tissue lysate. CD86 was used as a marker of M1-type MG/MΦ, and CD206 was used as a marker of M2-type MG/MΦ. β-actin was used as a cytoplasmic marker (n = 3). (A) and (B) One day after ROSC. (C) and (D) Three days after ROSC. (E) The frequencies of MG/MΦ expressing CD86 and CD206 1 day and 3 days after ROSC were measured by flow cytometry. Q1 indicates the rate of CD86⁺ cells, Q2 represents the cell rate in which both CD86⁺ and CD206⁺ are positive, and Q2 indicates the rate of CD206⁺ cells (n = 4). (F) The ratio of CD86⁺/CD206⁺ (n = 4) ^nsp > 0.05; *** p < 0.001; **** p < 0.0001