Fig. 4

Transferred mitochondria in MG/MΦ increased the number of mitochondria in hippocampus, and improved the morphology and structure of mitochondria in MG/MΦ. (A) Immunofluorescence analysis of mitochondria in hippocampal tissue. Green-stained cells are MG/MΦ, blue-stained regions are nuclei, and red arrows indicate the Mito-tracker Red CMXRos labeled exogenous mitochondria (400×, n = 3). (B) Immunofluorescence analysis of mitochondrial number. Blue-stained regions are nuclei, and red-stained regions are mitochondria in the hippocampus (400×, n = 3). (C) The mean gray value of TOMM20. (n = 3). (D) Morphology and structure of mitochondria in MG/MΦ were observed by electron microscopy. The red arrows indicate mitochondria; (scale bar 500 nm, n = 3); ^nsp > 0.05; *** p < 0.001