Fig. 4
Assay of AD-MSC proliferation and comparison of CFUs generated by AD-MSCs from sub-groups a and b. A Cell number (mean ± SD) was determined at the end of each passage (P1, P2, and P3) and presented as cell number/cm2. Cells from both sub-groups were plated using the same seeding density (6000 cells/cm2) and sub-cultured for 3 passages (7 days/passage). B Cell doubling time (mean ± SD) was calculated at the end of each passage. **P < 0.05 (n = 5), vs. sub-group b at the same passage. C Images of CFU-F, CFU-OB and CFU-AD formed by P1 and P3 AD-MSCs; 5 patients (numbers of 1–5) from sub-group a (ICRS score of I) and group b (ICRS score of III–IV) were analyzed to perform the comparison. All CFU assays for each patient were performed in triplicate. D Numbers of CFU-F, CFU-OB, and CFU-AD per well were performed in triplicate for each patient. The data shown consist of 15 values (3/patient with 5 patients/sub-group). **P < 0.05 (n = 15) vs. sub-group b based on mean ± SD at the same passage