Table 1 The primer sequence of all genes for RT-PCR
Gene | Forward | Reverse |
|---|---|---|
CD31 | CACCGTGATACTGAACAGCAA | GTCACAATCCCACCTTCTGTC |
VEGFA | CCCTGGCTTTACTGCTGTAC | TCTGAACAAGGCTCACAGTG |
OCN | CCTCACACTCCTCGCCCTATT | CCCTCCTGCTTGGACACAAA |
RUNX2 | ACTTCCTGTGCTCGGTGCT | GACGGTTATGGTCAAGGTGA |
miR-21-5p | TCGCTCGAGATTTTTTTTTATCAAGAGGG | TCGGCGGCCGCGACAAGAATGAGACTTTAATC |
NOTCH1 | TCGGAGTGGACAGGTCAGTA | AGATACACGCATCGTTCAGG |
DLL4 | TGCGGATAACCAACGACG | GCCCACAAAGCCATAAGGAC |
U6 | TCGGCGGCCGCGACAAGAATGAGACTTTAATC | CGCTTCACGAATTTGCGTGTCAT |
β-actin | GAGAGGGAAATCGTGCGTGA | GCCTAGAAGCATTTGCGGTG |