Fig. 5

Effect of intestinal flora on inflammatory factors and immune cell infiltration

A-E: TNF-α, COX-2, MMP9 and CTNNB expression levels in tumor tissues of mice in each group (n = 3) were detected using qRT-PCR (A-D) and western blot (E). F: E-cadherin, N-cadherin, Vimentin, Snail and CD133 levels in intestinal tissues of mice in each group (n = 3) were detected using Western blot. G: Spleen/body weight ratio of mice in each group (n = 6). H-I: Flow cytometry to detect changes in Th1 (CD4 + IFN-γ) and Th17 (CD4 + IL-17 A) cell numbers in the spleens of mice in each group (n = 3)

* P < 0.05, ** P < 0.01, vs. the NC mice. ## P < 0.01, vs. HC-FMT mice