Fig. 2

Higher intra-tumoral relative abundance of Bifidobacteriaceae and lower microbiome alpha diversity in patients with cervical cancer are significantly associate with strong immune infiltration. (A) Immunohistochemical (IHC) staining of CD8 and GzmB from tumors of IR and SR group patients (representative image, 200× magnification) (left) and quantification of IHC signal of CD8⁺, and GzmB⁺ from tumors of IR and SR group patients (right). Scale bar, 50 μm. (B) Spearman correlation between CD8⁺ and GzmB⁺ tissue densities and TVRR. (C-E) Spearman correlation between CD8⁺ and GzmB⁺ tissue densities and (C) Bifidobacteriaceae, (D) Beijerinckiaceae, (E) and Orbaceae in cervical cancer patients. (F) Spearman correlation between ACE, Pielou and Shannon indexes and TVRR in cervical cancer patients. (G) Spearman correlation between CD8⁺ and GzmB⁺ tissue densities and ACE, Pielou and Shannon indexes in cervical cancer patients. (H) The correlations of TVRR, CD8⁺, GzmB⁺, Bifidobacteriaceae, Beijerinckiaceae, and Orbaceae. (I) The correlations of TVRR, CD8⁺, GzmB⁺, ACE, Pielou and Shannon indexes. SR, superior response; IR, inferior response; GzmB, granzyme B; TVRR, tumor volume reduction rate