Fig. 5

VCE-004.8 induces eNOS phosphorylation and NO synthesis. A EA.hy926 cells were treated with VCE-004.8 for 3 h. peNOS protein expression is normalized to eNOS and results were expressed relative to control defined as 1. B VCE-004.8 induces NO production. EA.hy296 cells were seeded, preincubated with DAF-2DA and treated with sulforaphane (SFN) (50 µM) or VCE-004.8 (10 µM). The figure indicated the percentage of NO induction over untreated cells. The data passed the normality test performed by the Shapiro–Wilk test. P value is calculated using 1-way ANOVA followed by Tukey post hoc multiple comparisons to compare between the groups (n = 3 independent experiments per group). The results are shown as means ± SD. P value indicated in panel, significant as ****p < 0.0001. C VCE-004.8 induces phosphorylation in vascular endothelial cells in a B55α dependent manner. EA.hy296 cells were transfected with siB55α or scrambled siRNAs (siCTR) and 48 h later treated with VCE-004.8 for 3 h. Representative western blots of three independent experiments is shown. peNOS and B55α protein expressions are normalized to eNOS and actin respectively and the results were expressed relative to control defined as 1