Fig. 4

Correlation between TFF1 and S100P. A TFF1 displayed elevated expression in tumor cells compared to normal epithelial cells. B TFF1 exhibited increased expression in tumor cells compared to immune and stromal cells. C Multiplex immunofluorescence (MIF) performed on 20 non-small cell lung cancer (NSCLC) patients demonstrated the primary expression of TFF1 in tumor cells. D Significant correlation between TFF1 and S100P. E MIF performed on 20 NSCLC patients demonstrated the co-location of S100P and TFF1 in tumor cells. F Patients with high levels of both S100P and TFF1 had shorter overall survival (OS) time, and those with high S100P and high TFF1 displayed the worst clinical outcome. G Immunohistochemistry demonstrated elevated expression of S100P and TFF1 in tumor cells compared to normal epithelial cells