Fig. 4

MitoTraker Red staining (orange color), NucBlue staining (blue color) and metabolic HPLC analysis. (A) Computerized analysis of the MitoTracker fluorescence intensity on the Untreated versus Nicotine, IQOS and 1R6F after 6 h and 24 h of treatment. The figures presented are representative of at least three independent experiments. Scale bars in (A) 50 μm. (B) % cells intact mitochondria. (C) % of polarized cells. Effect of nicotine, IQOS and cigarette smoke treatment on levels of major classes of metabolites detected by HPLC (D-H). Values represent the mean ± SD of experiments performed in quadruplicate. (*p < 0.05, **p ≤ 0.01, ****p ≤ 0.0001)