Fig. 2

Effect of cigarette smoke on ROS cell-free production. (A) MFI (Mean Fluorescence Intensity) of ROS production after 6 and 24 h of treatment. (B) Membrane lipid peroxidation (LOOH) after 24 h of treatment. (C) GSH (reduced glutathione) levels after 24 h of treatment evaluated by HPLC analysis. (D) Nitrite and Nitrate levels after 24 h of treatment evaluated by HPLC analysis. Data are presented as the mean ± standard deviation of experiment performed in quadruplicate. (*p < 0.05; **p ≤ 0.01; ****p ≤ 0.0001)