Fig. 10

DNA damage measured by the comet assay. HMC3 cells were exposed for 24 h to nicotine, IQOS and 1R6F. (A) Representative fluorescence images of comets. (B) DNA double strand breaks (DSBs) were measured by neutral comet assay and expressed as TMOM. Data presented are median of ± 95% CI of experiments performed in quadruplicate. (*** p ≤ 0.001 and ****p ≤ 0.0001, as a result of the Kruskal-Wallis test followed by Dunnett’s post hoc test, denotes a significant increase in DSBs as indicated by the brackets for each specific comparison)