Fig. 1

HMC3 cells viability. (A) Cell viability according to bromide 3-(4,5 dimethylthiazol − 2-yl)-2,5-diphenyltetrazolium (MTT) assay after treatment for 24 h at different puffs/ml of cigarette smoke. (B-C) Inhibition of cell viability (IC50). (D) Real-time cell proliferation of HMC3 cells after cigarette smoke treatment (normalized cell index). (E) Area under curve. All assays were performed in quadruplicate. (*p < 0.05, ***p ≤ 0.001, ****p ≤ 0.0001)