Fig. 4

The protein expression of molecules associated with epithelial barrier in Irf7^−/− mice after DSS treatment. WT and Irf7^−/− mice were administered 3% DSS, and subsequently, sera and colons were collected on days 0 and 8. Colonic samples were collected, extracted and tested for protein levels of Muc2, E-cadherin, β-catenin and Occludin by Western-blot (A–E) and Immunofluorescence analysis (F). Detection of goblet cells by PAS staining (G). Scale bar, 50 µm. D0: WT (n = 4), Irf7^−/− (n = 4), D8: WT (n = 5), Irf7^−/− (n = 5). The experiment was independently repeated two times. The data are expressed as mean ± s.e.m. NS, not significant, **P < 0.01, ***P < 0.001, ****P < 0.0001. Two-way ANOVA with Holm-Sidak's multiple comparisons test (B–E)