Fig. 5
From: Effect of the mRNA decapping enzyme scavenger (DCPS) inhibitor RG3039 on glioblastoma
DCPS inhibition reduced STAT5B expression in GBM cells
(a–b) GBM cell lines were treated with RG3039 (6 µM) or DMSO for 48 h and then subjected to RNAseq. The top 20 differentially expressed transcription factors were displayed in heatmap (a) and volcano plot (b). FC, fold change
(c) Correlation analysis of the expressions of DCPS and STAT5B in GBMs in TCGA database. Statistical analysis by linear regression analysis
(d) GBM cell lines were transfected with siRNA targeting DCPS for 48 h. The expression of STAT5B mRNA was analyzed by RT-qPCR and normalized to that of β-actin (mean ± SEM, n = 3). Statistical analysis by unpaired t-test, **, p < 0.01, ***, p < 0.001
(e) GBM cell lines were treated with RG3039 (6 µM) or DMSO for 48 h. The expression of STAT5B mRNA was analyzed by RT-qPCR and normalized to that of β-actin (mean ± SEM, n = 3). Statistical analysis by unpaired t-test, ****, p < 0.0001
(f) GBM cell lines were transfected with siRNA targeting DCPS for 48Â h. The expressions of DCPS and STAT5B were analyzed by western blotting
(g) GBM cell lines were treated with RG3039 (6 µM) or DMSO for 48 h. The expression of STAT5B was analyzed by western blotting