Fig. 6

Suppression of LIN9 expression diminishes VSMC proliferation and migration, and reinstates sensitivity to PTX. A, B VSMCs were transfected with siRNA for 24 h and then treated with IL-1β. The protein and RNA expression levels of LIN9 were measured (n = 3). C After transfection with LIN9 siRNA, VSMCs were treated with different doses of PTX for 24 h, and the changes in the inhibitory efficiency of PTX were observed (n = 6). D Cell scratch assay was performed to analyze the inhibitory effect of LIN9 knockdown on IL-1β-induced cell migration and the modulation of cell sensitivity to PTX (scale bar: 200 μm; n = 3). E CCK-8 assay was conducted to evaluate the inhibitory effect of different doses of JQ1 on the IL-1β-induced proliferation of SMCs. The cells were first treated with IL-1β (10 ng/mL) for 24 h, followed by treatment with varying doses of JQ1 for an additional 24 h (n = 6, ###, p < 0.001, versus control group; ***, p < 0.001, versus IL-1β treatment group). F Flow cytometry analysis was performed to assess the effect of JQ1 on the cell cycle and to evaluate the modulation of cell sensitivity to PTX by JQ1 (n = 5). G CCK-8 assay was conducted to analyze the restoration of SMC sensitivity to PTX by JQ1 (n = 6). H Western blot and qRT-PCR experiments were performed to analyze the effects of different treatments on the protein and RNA expression levels of LIN9 (n = 3). Data are presented as mean ± SEM (*p < 0.05, **p < 0.01, ***p < 0.001)