Fig. 5

IL-1β recruits BRD4 to upregulate LIN9 expression in SMCs. A IHC experiments were conducted to validate the differential expression of LIN9 in femoral artery tissue, (scale bars: HE, 200 μm; IHC, 100 μm; n = 6 per group). B, C Protein and mRNA of LIN9 extracts from femoral artery tissue were used to perform western blotting and qRT-PCR experiments, (N means sample ID; n = 6 per group). D, E VSMCs were treated with IL-1β alone or in combination with PTX, and then examined using western blotting and qRT-PCR to observe the changes in BRD4 and LIN9 expression (n = 3). F Fluorescence co-localization experiments were performed to further investigate the interaction between BRD4 and LIN9 (DAPI, blue; BRD4, green; LIN9, red), images were acquired using confocal microscope, ( scale bar 10 μm). G The interaction between BRD4 and LIN9 within the cells was examined using immunoprecipitation (IP) assay. (H) In the PLA experiment, the interaction between BRD4 and LIN9 was observed (DAPI, blue; PLA spots, red; scale bar 50 μm). Data are presented as mean ± SEM (*p < 0.05, **p < 0.01, ***p < 0.001)