Fig. 2

Albendazole attenuates the aerobic glycolysis in TGF-β1 treated fibroblast. A ECAR was recorded in fibroblasts after the addition of glucose (10 mM) oligomycin (1 µM) and 2-DG (50 mM). B Bar graph of the basal levels of ECAR. C OCR was determined in fibroblasts after the addition of oligomycin (1 µM) FCCP (0.5 µM) and rotenone/antimycin A (0.5 µM). D Bar graph of the basal levels of OCR. E Mean ratios of ECAR/OCR. F Relative levels of lactate in the supernatant of fibroblasts. G WB analysis of PFKFB3, HK2, LDHA, and PKM2 expression in fibroblasts. Left side: Representative image of WB data. Right side: Bar chart depicting WB data. H Immunofluorescence of PFKFB3 in fibroblasts. The data are represented as the mean ± SEM of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001