Fig. 6
From: An oncolytic HSV-1 vector induces a therapeutic adaptive immune response against glioblastoma
Immunohistological characterization of tumor microenvironment in GL261 C57BL/6 bearing mice treated with oHSV-1 compared to untreated mice. IHC staining of serial brain sections. Slides of brain tissues isolated from oHSV-1-injected GL261 tumors and challenged with GL261 are indicated in the upper and middle panels, respectively. Slides of brain tissues isolated from GL261-untreated mice are represented in the lower panels (vehicle). Sections were stained by IHC with anti-CD11b, anti-CD68, anti-IBA1 and anti-GFAP antibodies. Images were taken at 200x magnification. Black and red aarrowheads in the IBA-1-stained upper and middle panels point to ameboid and branched macrophages, respectively. IBA-1: Ionized calcium-binding adapter molecule 1, GFAP: glial fibrillary acidic protein. Scale bar corresponds to 50 μM