Fig. 4

Differential expression of SPP1 in normal versus tumor tissues. (A): Analysis of SPP1 expression differences using the TCGA database. (B): RT-PCR revealed expression variations of SPP1 between HCC tumor cells and normal hepatocytes (n=3). (C-D): WB assessed SPP1 expression differences in HCC tumor cells versus normal hepatocytes (C) and between HCC and normal tissues (D) (n=3). (E): IHC analyzed SPP1 expression in 20 matched pairs of HCC and normal tissues (n=20). (F): ELISA measured differences in OPN content in CM from normal and tumor hepatocytes (n=3). (G): TCGA database analysis explored the correlation between SPP1 expression and patient prognosis. *p<0.05, **p<0.01, ns: p>0.05