Fig. 3

Single-cell sequencing analysis of SPP1 expression in relation to CAF infiltration. (A): Annotated UMAP clustering map for single-cell objects. (B): Identification of classical marker gene expression levels across various cell types. (C): Bar chart showing the percentage of cell types within the 12 samples in a single-cell object. (D): Violin plots displaying SPP1 expression in different cell types. (E): Distribution of SPP1 expression on UMAP projection. (F): Mean percentage of fibroblasts in the SPP1 high expression group versus the SPP1 low expression group. (G): Cell pairs with ligand-receptor interactions across different cell types. (H): The top 20 significant ligand-receptor pairs between tumor cells and fibroblasts. (I): EPIC algorithm fitted to the Correlation curves between SPP1 expression (vertical axis) and the degree of fibroblast infiltration (horizontal axis) in the TCGA hepatocellular carcinoma database. *p<0.05, **p<0.01, ns: p>0.05