Fig. 2

SPP1 is primarily secreted by tumor cells and exhibits various ligand-receptor relationships with CAFs. (A): Average SPP1 expression levels of different cell types in the cHC spatial transcriptomic sample. (B): Spatial distribution of different cell types in the cHC spatial transcriptomic sample. (C): Distribution of different cell types in the umap dimensionality reduction space. (D): Average SPP1 expression levels of different cell types in the HCC5C spatial transcriptomic sample. (E): Spatial distribution of different cell types in the HCC5C spatial transcriptomic sample. (F): Distribution of different cell types in the umap dimensionality reduction space. (G): Heatmap showing the correlation between SPP1 expression levels of spots and enrichment scores of 26 cell types in 10 spatial transcriptomic samples. (H): Correlation analysis of SPP1 expression levels and tumor cell scores in HCC5A samples. (I): Spatial distribution between the highest expressing SPP1 malignant tumor subpopulation and CAFs in the HCC2T sample. (J): Cell communication analysis between tumor cells and CAFs selected from Fig. 2I. (K): Bubble chart showing the top 20 significant ligand-receptor interactions between the two cell populations in Fig. 2I. (L): Spatial distribution between the highest expressing SPP1 malignant tumor subpopulation and CAFs in the HCC2P sample. M: Cell communication analysis between tumor cells and CAFs selected from Fig. 2L. (N): Bubble chart showing the top 20 significant ligand-receptor interactions between the two cell populations in Fig. 2L. *p<0.05, **p<0.01, ns: p>0.05