Fig. 1

scRNA-seq analysis of PBMCs of patients who underwent allo-HSCT revealing immune regulation signature in aGVHD. A Unsupervised clustering (UMAP plot) of scRNA-seq data from PBMCs of patients with aGVHD and with non-aGVHD, with cell subtypes labeled. B The frequency percentage of the main cell subtypes (patients with non-aGVHD, n = 6; patients with aGVHD, n = 6). C Dot graph illustrating the GO analysis of upregulated genes in PBMCs of patients with non-aGVHD compared with those from individuals with aGVHD. D Dot plots illustrating relative expression levels of the upregulated genes IRF7, IRF1, NLRC4, LILRB1, LILRB2, and LILRB4 in PBMCs of patients with non-aGVHD. E Dot graph illustrating the GO analysis of upregulated genes in PBMCs from patients with aGVHD compared with those from individuals with non-aGVHD. F Dot plots were used to display the relative expression levels of downregulated genes (JUNB, FOS, JUN, CD83, and EGR1) in PBMCs of patients with non-aGVHD. G Representative immunofluorescence staining of c-FOS, c-JUN, IRF7 and CD45 in small intestine of control or aGVHD mouse model groups. Scale bar, 20 µm