Fig. 6
From: miR-876-3p is a tumor suppressor on 9p21 that is inactivated in melanoma and targets ERK
Effects of regulation of ERK by miR-876 on melanoma colony formation and drug sensitivity. A, B Western analysis of ERK expression in C8161.9 (panel A) and A375 (panel B) cells stably expressing miR-876 transfected with a plasmid encoding MAPK1/ERK2 cDNA or empty vector. C, D Bar graphs showing the average number of colonies in C8161.9 (panel C) and A375 (panel D) cells stably expressing miR-876 transfected with a plasmid encoding MAPK1/ERK2 cDNA or empty vector, with accompanying representative bright field images of C8161.9 and A375 colonies. E Viability of A375 cells expressing miR-876 or control miR following treatment with various concentrations of vemurafenib. Data are shown as the mean ± SEM. *P < 0.05 denotes statistically significant differences compared with control