Fig. 5
From: miR-876-3p is a tumor suppressor on 9p21 that is inactivated in melanoma and targets ERK
ERK as a potential target of miR-876. A The putative binding sites of miR-876 in the 3′UTR of MAPK1/ERK2. B, C Luciferase reporter assays verifying the regulation of miR-876 in the 3’UTR of MAPK1/ERK2 in C8161.9 (panel B) and A375 (panel C) cells transiently co-transfected with miR-876 (or control-miR) and a plasmid encoding the luciferase reporter gene and MAPK1/ERK2 3’ UTR. D, E Western blot analysis of ERK expression in C8161.9 (panel D) and A375 (panel E) expressing miR-876 vs control-miR