Fig. 4

GSVA enrichment analysis in Hallmarks and functional genesets specific to OSCC. Heatmap plot representing the value of GSVA score calculated for each type of ROI, belonging to 4 groups of patients. A Lymph node-positive–tumor end, lymph node positive –stromal end, B Lymph node negative–tumor end and lymph node-negative- stromal end). Gradient-wise change in the regulation status of the Hallmark genesets is observed as the distance between tumor and immune cells increases. Positive GSVA scores represent genes in the HALLMARK gene set that are positively enriched while negative GSVA scores represent genes in the HALLMARK gene set that are negatively enriched. Gradient-wise change in the regulation status of the OSCC-specific expression programs can be observed as the distance between tumor and immune cells increases. C Lymph node-positive–tumor end, lymph node positive–stromal end, D Lymph node negative– tumor end, and lymph node-negative- stromal end. Positive GSVA scores represent genes in the expression program that are positively enriched while negative GSVA scores represent genes in the expression program that are negatively enriched