Fig. 3

Fate determination and transcriptional regulation mechanisms of gastric cancer CD8⁺ T cells differentiation. A Monocle3 analysis identifying the differentiation trajectory of CD8⁺ T cells, with colors corresponding to pseudotime. B Along the pseudotime axis, density distribution plot of CD8⁺ T cells subpopulations by position. C Dynamic visualization of immune checkpoint expression along the pseudotime analysis axis. D Scatter plot illustrating the transcriptional regulatory network of (left) CD8⁺ Tex subpopulation and (right) CD8⁺ KLRC1⁺ Trm subpopulation. E Box plot showing the expression of transcription factors MSC and RUNX3. F CellOracle simulation depicting the cell fate vector plot after (left) MSC and (right) RUNX3 knockout; *P-value < 0.05; **P-value < 0.01; ***P-value < 0.001; ****P-value < 0.0001