Fig. 2

Projection of unsorted BM cells on the BM cell reference describes the connection between MDS and AML. A Density plot of 4 healthy donors on the BM cell reference map using MarcoPolo working flow (See the text and Methods for details; The cropped reference map shows 8 major cell types as indicated (left panel). A balanced density plot is visualized for each of the four healthy donors (right panel). Notice high density of cells in the highlighted region (dashed line). B Density plot of 5 MDS patients. 4 out of 5 MDS demonstrate very low cells in the highlighted region. High density of pDC in MDS1 is also noticed (pink arrow). Please also notice the abnormal high density of GMPs in the MDS2, 5 and 4 (yellow arrows). C Density plot of an MDS patient from the study GSE205490. D Density plot of a healthy donor and 3 AML patients from the study GSE130756. E Quantitation of fractions of indicated cell types in the unsorted bone marrow cells based on the values generated by the MarcoPolo pipeline. F The ratio of myeloid/lymphoid cells based on the values generated by MarcoPolo pipeline.*, p < 0.05; **, p < 0.01