Fig. 1

scRNA-Seq analysis of unsorted BM cells revealed hematopoietic abnormalities in the MDS patients. A Five MDS patients and four healthy controls donated the bone marrow biopsies for the single cell RNA-sequencing (scRNA-seq) study. See Table 1 for clinical information of the MDS patients. B In total 14 cell types were annotated in the UMPA plot. NK, nature killer cells; T, T cells; B, B cell; GMP, granulocyte–macrophage progenitor cells; MEP, megakaryocyte-erythroid progenitors; Eryp, erythroid progenitors; pDC, plasmatic dendric cell. C Bar plot for fraction of each cell type in the samples. The cell numbers of each sample are shown on the top of the panel. Notice that the high-risk MDS group has a much less fraction of pro-neutrophils, neutrophils and monocytes. D Quantitation of fractions of indicated cell types in the unsorted bone marrow cells. E The ratio of myeloid/lymphoid cells. n.s. not significant; *, p < 0.05; **, p < 0.01