Fig. 3
miR-145a-5p overexpressed M2 microglia transplantation improved the locomotor function of SCI mice. (A) Mouse movement behaviors on a plank were photographed in different group including sham, SCI, SCI with M2 + oligo microglia delivery, and SCI with miR-145a-5p overexpressed M2 microglia delivery after 28 days. (B, C) The Basso mouse scale (BMS) score (B) and subscore (C) in (A) were analyzed and quantitatively compared (n = 6). (D) Mouse swimming in water in different group was recorded. (E) LSS score in (D) was statistically analyzed (n = 6). (F) hindlimb reflex scoring was statistically analyzed (n = 6). Data shown as mean ± SEM, *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001, SCI mice with M2-miR-145a-5p microglia treatment versus SCI mice without treatment. #, p < 0.05; ##, p < 0.01; ###, p < 0.001; ####, p < 0.0001, SCI mice with M2 microglia treatment versus SCI mice without treatment. &, p < 0.05; &&, p < 0.01; &&&, p < 0.001; &&&&, p < 0.0001, SCI mice with M2-miR-145a-5p microglia treatment versus SCI mice with M2 microglia treatment. Statistical analysis was performed using a two-way repeated ANOVA with Bonferroni’s post hoc test