Fig. 1
miR-145a-5p promoted M2 while inhibited M1 microglia polarization leading to the reduced oxidative stress-induced neuron damage. (A) BV2 cells were transfected with miR-145a-5p mimics or Ctrl and stimulated with PBS, LPS + IFN-γ or IL-4 for 24 h, respectively. The mRNA level of M1 markers including TNF-α, IL-6, IL-1β and iNOS, as well as M2 markers containing Arg1, Mrc1, YM1 and IL-10 were determined by qRT-PCR, respectively (n = 5). (B) BV2 cells were transfected with miR-145a-5p ASO or Ctrl and treated as (A). The mRNA level of M1 and M2 markers were determined by qRT-PCR, respectively (n = 5). (C, D) BV2 cells were treated as (A), and then collected and lysed. The protein level of iNOS and Arg1 were detected by Western blot. Representative image (C) and quantitative data (D) were shown (n = 4). (E, F) BV2 were treated as (B), and then collected and lysed. The protein level of iNOS and Arg1 were detected by Western blot. Representative image (E) and quantitative data (F) were shown (n = 4). (G, H) The supernatant of BV2 cells treated as (A) was collected, and then co-cultured with H2O2-treated hippocampal neurons. The activated cleaved-caspase 3 of neuron was determined by Western blot. Representative image (G) and quantitative data (H) were shown (n = 4). Data shown as mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001 by unpaired student’s t-test. ASO, antisense oligonucleotides