Fig. 6

Left side) A schematic design for the development of hydrogels and their potetnail in the cargo release in response to light. Right side) (a) The anti-cancer activity of doxorubicin-loaded hydrogels in vitro against BT-20 cells in the presence and lack of irradiation (4-watt, 5 min). Based on the figure, the application of irradiation causes more decrease in the viability of cells compared to the lack of irradiation. Moreover, DOX-loaded HACOUM-50 demonstrates better anti-cancer activity compared to DOX-loaded HACOUM-100, decreasing viability of cells to less than 60%. (b) FOBI images of hydrogels achieved after excitation at different wavelengths of light, and (c) fluorescence spectrophotometer curves of COUM-OH intermediate and DCOUM-PEG-DTz obtained after excitation at various wavelengths of light. Upon excitation at 400 nm, the hydrogels showed a favourable fluorescent emission peak at 473 nm. Upon excitation at 600 nm, there were two emission peaks including 303 and 401 nm.