Fig. 5

Spleen Staining, Levels of Aging-Related Factors in the Spleen, and Peripheral Blood CD4 + T/CD8 + T Ratio in Different Rat Groups. (A) HE staining of the spleen in various rat groups, scale bar = 100 μm. (B) Tunel staining of the spleen in different rat cohorts, scale bar = 100 μm. (C) PCNA staining of the spleen in various rat groups, scale bar = 100 μm. (D) Immunohistochemical staining for p16 in the spleens of different rat cohorts, scale bar = 100 μm. (E) Immunohistochemical staining for p21 in the spleen across various rat groups, scale bar = 100 μm. (F) Semi-quantitative analysis of Tunel staining in the spleen (n = 5). (G) Semi-quantitative analysis of PCNA staining in the spleen (n = 5). (H) Immunohistochemical scoring for p16 and p21 in the spleen (n = 5). (I) CD4 + T/CD8 + T cell ratio in peripheral blood of young control group, aged treatment group pre-treatment, and aged treatment group post-treatment (n = 5). (J) CD4 + T/CD8 + T cell ratio in peripheral blood of different rat groups (n = 5). (K) Levels of aging-related factors in the spleen, including β-gal, lipofuscin, SOD, MDA, GSH, IgG, IL-1β, and IL-6 (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001