Fig. 4

Staining of Rat Brain Tissues and Levels of Aging-Related Factors. (A) HE staining of the cerebral cortex in different rat groups, scale bar = 100 μm. (B) Nissl staining of the hippocampal region of various rat cohorts, scale bar = 100 μm. (C) Immunohistochemical staining for p16 in the hippocampal region of different rat groups, scale bar = 100 μm. (D) Immunohistochemical staining for p21 in the hippocampal area of various rat cohorts, scale bar = 100 μm. (E) Semi-quantitative analysis of Nissl staining in the hippocampal region (n = 5). (F) Immunohistochemical scoring for p16 in the hippocampus (n = 5). (G) Scoring of p21 immunohistochemistry in the hippocampal region (n = 5). (H) Levels of aging-related factors in brain tissues of different rat groups, including β-gal, lipofuscin, SOD, MDA, GSH, IgG, IL-1β, and IL-6 (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001