Fig. 2

A simplified ex vivo culture system was developed to evaluate the synergy between NB73 and Venetoclax in primary multiple myeloma cells. (A) Illustration of simplified ex vivo culture for primary MM cells without cytokine supplementation. (B) After 18 h, primary MM cell viability remained at 70% compared to baseline. (C) Drug synergy between NB73 and Venetoclax was observed in three cases post 18-hour ex vivo culture. (D) No observed drug synergy between NB73 and Venetoclax in three cases following 18-hour ex vivo culture. (E) Relative RNA levels of FOXM1 and BCL2 were assessed using qRT-PCR in the seven cases