Fig. 5

Immune subtype identification. A Unsupervised clustering of immune cells clustered the CRC samples into 3 subtypes. The C3 and C1 subgroups are highlighted with a rectangle. B Heatmap showing the correlation of the immune phenotypes. C KM plot for OS-related survival of the identified immune clusters. D-E Representative CD20- and CD3-stained images show the lymphoid follicles in the tumor mass D and invasive front (E). Ki-67 and PANCK are shown to aid in the identification of cancer cells. F Overall survival plot for the TME compartment Tregs in C1 and C3