Fig. 3
BIC suppresses SiO2-induced macrophage polarization, proliferation and apoptosis. RAW264.7 cells were pretreated with SiO2 (100 µg/mL) for 8 h, followed by treatment with different concentrations of BIC (0, 50, 100,150, and 200 nmol/L) for 48 h. The parental group was not stimulated with either SiO2 or BIC. A. Flow cytometry analysis (up) and statistical ratios of M1 and M2 polarized macrophages (down) in SiO2-stimulated RAW264.7 cells treated with different concentrations of BIC. B. Apoptosis (up) and statistical analysis (down) of the apoptotic percentage of SiO2-stimulated RAW264.7 cells detected via flow cytometry, using Annexin V-FITC/PI staining. C. Western blot analysis of the expression of apoptosis-related proteins in SiO2-stimulated RAW264.7 cells treated with different concentrations of BIC. β-Actin was used as a loading control. All data are presented as mean ± SEM. Histograms in this figure share common symbols (blue for parental, red for 0 nmol/L, green for 50 nmol/L, purple for 100 nmol/L, orange for 150 nmol/L, and black for 200 nmol/L BIC treatment after SiO2 stimulation). *p < 0.05, **p < 0.01, and ***p < 0.001 compared to the 0 nmol/L group