Fig. 7

AKAP12/LMOD1 overexpression inhibits the malignant phenotype of CRC cells. A A volcano plot displaying the differentially expressed mRNAs in AKAP12 knockdown cells [search in genetic perturbation similarity analysis database (GPSAdb), accession: GSE147739]. LMOD1 was marked in a red box. B Association of AKAP12 expression with LMOD1 in TCGA pan-cancer. Grey = not statistically significant, Blue = negatively correlated, and Red = positively correlated. A spearman test was carried out. C Specific spearman correlation coefficients between AKAP12 and LMOD1 in TCGA-COAD (left) and TCGA-READ (right) are shown. D DEGs enrichment analysis after AKAP12 knockdown in GSE147739. The larger the circle, the higher the number of genes, and the smaller the P-values, the darker the color and the more significant the enrichment. E Gene set enrichment analysis (GSEA) plots of TGF_BETA_BETA signals were analyzed in GPSAdb (accession: GSE147739). F Association of AKAP12 expression with TGF_BETA_BETA signals level in TCGA pan-cancer. Blue = negatively correlated, Red = positively correlated, and Grey = not statistically significant. A spearman test was carried out. G, H The wound-healing assays for ROK and SW480 cells over-/under-expressing AKAP12 in the presence or absence of si-LMOD1 (Magnification, × 200, scale bars = 100 μm). I, J Transwell migration assays for ROK and SW480 cells over-/under-expressing AKAP12 in the presence or absence of si-LMOD1 (Magnification, × 200, scale bars = 100 μm). K, L The expression of proteins related to EMT in RKO and SW480 cells was determined by western blotting (n = 3 replicates). Data are presented as the mean ± SEM. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05. All assays were performed thrice, independently