Fig. 5

Effect of ALYREF on cell proliferation and angiogenesis. (A) An EdU assay was performed to detect the effect of si-ALYREF on cell proliferation. (B) A CCK-8 assay was performed to detect the effect of si-ALYREF on cell proliferation. (C) Western blot analysis of the changes in cyclin D1 protein levels after si-NSUN2 transfection. (D, E) Effect of HG and si-ALYREF on the tube formation ability of HRMECs was examined by tube formation assay. (F) Western blot was used to measure the changes in CD31 and VEGFA protein levels after si-ALYREF