Fig. 3

Microbial mutational patterns and signature alternation during evolution. (A) Comparison of mutational signatures based on base substitutions and Ti\Tv between the four groups. (B) Differential microbial mutational signature contribution associated with CC progression. (C) Abundance of snv with base mutation types C > T and T > C in CC, CIN, HPVi and control groups. (D) Characterization of 96 mutations in CC, CIN, HPVi and control groups based on the reference genome of bacteria. The base substitution at the mutation site contains six types: C > A, C > G, C > T, T > A, T > C and T > G. Four bases (A, T, C, G) can be paired on each side of the mutation site (5′ and 3′ ends), resulting in 96 possible mutation types (6 base substitution types at the mutation site × 4 5′ bases × 4 3′ bases). CC, cervical cancer; CIN, cervical intraepithelial neoplasia, HPVi, Human papilloma virus infection; Ti/Tv, the ratio of transitions to transversions for the pair of sequences