Fig. 9

API modulated the expression of proteins involved in mitochondrial dynamics. (A) Western blot analysis showed that API downregulated DRP1 expression at 12 and 24 h after RIR. (B) DRP1 expression gradually decreased from day 1 to day 7. (C) Western blot analysis demonstrated that API upregulated DRP1 expression at day 7 after RIR. (D, F) Expression of the mitochondrial fusion proteins OPA1 and MFN2 decreased from day 1 to day 7. (E, G) Western blot analysis showed that API upregulated OPA1 and MFN2 expression at day 7 after RIR. (H-I) Expression levels of OPA1, MFN2, and DRP1 were compared with normal values at different time points and in different groups. N = 3 in each group. Data are shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. OPA1, optic atrophy 1; MFN2, mitofusin-2; DRP1, dynamin-related protein-1