Fig. 2

MSCs in the leukemia niche showed altered gene expression profiles. A The distributions of gene expression in MSCs cultured alone and MSCs co-cultured with Nalm-6 for 72 h based on log₁₀(RPKM), (n = 3 independent experiments). B Correlation analysis of MSCs cultured alone and MSCs co-cultured with Nalm-6 by HeatMap diagram. C Principal component analysis of MSCs cultured alone and MSCs co-cultured with Nalm-6. D and E The heatmap (D) and volcano plot (E) of gene expression in MSCs cultured alone and co-cultured with Nalm-6 based on -log₁₀(FDR) (MSCs + Nalm-6 vs MSCs). F GO-Biological Process analysis of total differentially expressed genes (Total), upregulated genes (UP) and downregulated genes (Down) in MSCs co-cultured with Nalm-6 group compared to MSCs cultured alone group. G and H GSEA of differentially functional gene subsets in MSCs co-cultured with Nalm-6 (G) and MSCs cultured alone (H)