Fig. 3

sCLU regulated the cancer stem cell properties of HCC cells. a The representative images of tumor spheroids derived from HepG2 and HCCLM3. b sCLU expression in attached cells and spheroids was detected by western blotting. c sCLU mRNA expression in attached cells and spheroids was detected by RT-qPCR. d Tumor spheroids derived from HepG2 cells transfected with NC or OE-sCLU were exposed to sorafenib solution. e The mean size of spheroids in d. f Tumor spheroids derived from HCCLM3 cells transfected with NC or sh-sCLU were exposed to sorafenib solution. g The mean size of spheroids in f. **P < 0.01